P 185 Recurrent bilateral keratoconus after corneal transplantation

w ED1 + lmacmphages, monocytes and dendritic cellsl and ED2+ cells ImacrophaQoa ordvl agpear to have a role in comeal rejection followinp pen&ti~ kerato&~~ in the rat. In the fdlowinp presentation the distribution of ED1 and ED2 wsitive cdls in normal weal tissues of Lewis rats, the influence of comeal transpiantation and the efIeci of Cwlnspuin A lCsAl upon both types of wllr was examined. m 66 female Lewis rats ware divided into following groups: Ill operated, untreated; ll l l operated, CsA tmated llOmg/kg i.m.1; Ill11 unoperated, untreated; IIVl unogerated, CsA treated llChng/kQ i.m.1. The animals in group ill and lll l received cornea1 buttons from Lewis-bDwn NOTway rats, who differ completely for mayor histommpatabilii complex. From wr previous studies dw rejection time of group ill is 13 days, of group (II) 16 days. Animals of group ill and (Ill wra killed on the 5th. 9th and 13th postoperatlva day, of group lll l also on day 16. Both operated and partner eve wre enucleated and immadiataly fixed in 100% alcohol. The iris. ciliarv bodv andchoroid wera excised as a whole or flat mount and the number and~dimibutian of ED1 and ED2 positive cells were obawvad uaing APAAP immunhistozhemistry. s In the normal iris (Ill) 4130 ED1 + cetIs/mm* and 400 ED2+ c&Is/mm’ could be found. Whik the number of ED1 + calls in the iris increased 3 times in value fdbwinp bmtodaatv, the number of EDZ+ cells remained stable. The number of w&l ED2: cells did not increase in either the operated or in the parmar ave. Although svatamic treatment with cvclosporin A resulted in a clinically significant delay in the cornad rejection progress, it did not effect the ED1 + and ED2 + cells. &&f&g& Psnatrating keratoplasty results in an increase of ELI1 + cells in the irio of tba ogerated aye. CM treatment doas not appear to significantly effect this cdl population following wwtrating keratoplasly. Uveal ED2+ cells were not influenced by comsal tmnsplsntation and CsA treatment.